FRACTIONATION OF THE TRIACYLGLYCEROLS OF LIPASE MODIFIED BUTTER OIL

Triacylglycerols (TGs) of lipase-modified butter oil were separated into saturated, monoene, diene and triene fractions on a p-propylbenzene sulfonic acid solid-phase extraction column loaded with silver ions. Fatty acid analysis of the fractions showed that the amounts of saturated TGs (98.4 mol%) and monoene TGs (26.0 mol%) in the saturated and monoene fractions, respectively, were close to the theoretical amounts of TGs in pure fractions. The column method provides a useful alternative to AgNO3-thin-layer chromatography as a means of separating the TGs of butterfat and producing relatively pure TG fractions for further analysis by gas chromatography (GC) or GC-mass spectrometry.