FILARIAL PARASITES CONTAIN A RAS HOMOLOG OF THE TC4/RAN/SPI1 FAMILY

被引：13

作者：

DISSANAYAKE, S

XU, M

PIESSENS, WF

机构：

[1] Department of Tropical Public Health, Harvard School of Public Health, Boston, MA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 56卷 / 02期

关键词：

FILARIAL PARASITES; GTP-BINDING PROTEINS; DEVELOPMENTAL REGULATION; EPIDERMAL GROWTH FACTOR; RAS PROTEINS; MITOTIC REGULATOR;

D O I：

10.1016/0166-6851(92)90175-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the 'ras-like' nuclear proteins TC4, ran and Spil. Rabbit antisera to bacterially expressed filarial protein detect a 24-22 kDa doublet in extracts of adult B. malayi and mature microfilariae, which is absent from immature microfilariae,. Increased expression of the native parasite protein occurs when worms are cultured in the presence of epidermal growth factor.

引用

页码：259 / 267

页数：9

共 30 条

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