MICROBORE COLUMNS - DESIGN, CONSTRUCTION, AND OPERATION

被引：63

作者：

REESE, CE ^{[1
]}

SCOTT, RPW ^{[1
]}

机构：

[1] HOFFMANN LA ROCHE INC,DEPT CHEM RES,NUTLEY,NJ 07110

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1980年 / 18卷 / 09期

关键词：

D O I：

10.1093/chromsci/18.9.479

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The dependence of column dimensions on the resolution required and the inherent apparatus dispersion are examined theoretically. The dependence of column length on reduced plate height is demonstrated, and the reduced plate height that can be expected from commercial columns is assessed experimentally. The overall extra-column band dispersion that takes place in three commercial chromatographs is experimentally determined. The dimensions of columns that can be satisfactorily used with each chromatograph are calculated. 22 Refs.

引用

页码：479 / 486

页数：8

共 22 条

[1]

Desty D. H., 1960, GAS CHROMATOGRAPHY 1, P162

[2] SAMPLE LOADING AND EFFICIENCY IN ADSORPTION, PARTITION AND BONDED-PHASE HIGH-SPEED LIQUID-CHROMATOGRAPHY [J].