DETERMINATION OF FLAVONOIDS AND ALKALOIDS IN THE SCUTE COPTIS HERB COUPLE BY CAPILLARY ELECTROPHORESIS

被引：66

作者：

LI, KL ^{[1
]}

SHEU, SJ ^{[1
]}

机构：

[1] NATL TAIWAN NORMAL UNIV,DEPT CHEM,TAIPEI,TAIWAN

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 313卷 / 1-2期

关键词：

CHROMATOGRAPHY; FLAVONOIDS; ALKALOIDS; HERB COUPLE;

D O I：

10.1016/0003-2670(95)00190-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A micellar electrokinetic capillary chromatographic (MEKC) method was developed for the simultaneous assay of six scute flavonoids (baicalin, wogonin 7-O-glucuronide, oroxylin A 7-O-glucuronide, baicalein, wogonin and oroxylin A) and four coptis alkaloids (berberine, palmatine, coptisine and epiberberine) in the scute-coptis herb couple. The carrier composed of a buffer solution (5 mM sodium berate, 15 mM sodium dihydrogenphosphate and 50 mM sodium cholate) and acetonitrile (3:2) was found to be the most suitable electrolyte for this separation, whereby the contents of these compounds in the herb couple and herb couple containing Chinese herbal preparations could be easily determined within 30 min. The effect of pH value, surfactant concentration and organic modifier (acetonitrile) concentration of the carrier on the migration and separation of the solutes were also studied.

引用

页码：113 / 120

页数：8

共 10 条

[1]

HSU HY, 1980, COMMONLY USED CHINES, P105

[2]

HSU HY, 1985, CHINESE MAT MED CONC, P112

[3] ALKALOIDS OF COPTIS RHIZOME [J].

IKUTA, A ;

ITOKAWA, H .

JOURNAL OF NATURAL PRODUCTS, 1984, 47 (01) :189-190

[4]

LIN CL, 1992, HERB COUPLES, P1

[5] DETERMINATION OF THE 6 MAJOR FLAVONOIDS IN SCUTELLARIAE RADIX BY MICELLAR ELECTROKINETIC CAPILLARY ELECTROPHORESIS [J].

LIU, YM ;

SHEU, SJ .

ANALYTICA CHIMICA ACTA, 1994, 288 (03) :221-226

[6] DETERMINATION OF QUATERNARY ALKALOIDS FROM COPTIDIS RHIZOMA BY CAPILLARY ELECTROPHORESIS [J].

LIU, YM ;

SHEU, SJ .

JOURNAL OF CHROMATOGRAPHY, 1992, 623 (01) :196-199

[7] STUDIES ON THE CONSTITUENTS OF SCUTELLARIA SPECIES .2. ON THE FLAVONOID CONSTITUENTS OF THE ROOT OF SCUTELLARIA-BAICALENSIS GEORGI [J].

TOMIMORI, T ;

MIYAICHI, Y ;

IMOTO, Y ;

KIZU, H ;

TANABE, Y .

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1983, 103 (06) :607-611

[8] STUDIES ON THE CONSTITUENTS OF SCUTELLARIA SPECIES .4. ON THE FLAVONOID CONSTITUENTS OF THE ROOT OF SCUTELLARIA-BAICALENSIS GEORGI .4. [J].

TOMIMORI, T ;

MIYAICHI, Y ;

IMOTO, Y ;

KIZU, H ;

SUZUKI, C .

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1984, 104 (05) :529-534

[9] ON THE FLAVONOID CONSTITUENTS FROM THE ROOTS OF SCUTELLARIA-BAICALENSIS GEORGI .1. [J].

TOMIMORI, T ;

MIYAICHI, Y ;

KIZU, H .

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1982, 102 (04) :388-391

[10]

TOMIMORI T, 1984, YAKUGAKU ZASSHI, V104, P524, DOI 10.1248/yakushi1947.104.5_524

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