DETERMINATION OF THE 6 MAJOR FLAVONOIDS IN SCUTELLARIAE RADIX BY MICELLAR ELECTROKINETIC CAPILLARY ELECTROPHORESIS

被引：53

作者：

LIU, YM ^{[1
]}

SHEU, SJ ^{[1
]}

机构：

[1] NATL TAIWAN NORMAL UNIV,DEPT CHEM,TAIPEI 117,TAIWAN

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 288卷 / 03期

关键词：

ELECTROPHORESIS; FLAVONOIDS; PHARMACEUTICALS; SCUTELLARIAE RADIX;

D O I：

10.1016/0003-2670(94)80134-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A capillary electrophoretic method for determining the six flavonoids in Scutellariae Radix, i.e., baicalin, baicalein, wogonin 7-O-glucuronide, wogonin, oroxylin A 7-O-glucuronide and oroxylin A, was developed. A buffer solution composed of 20 mM sodium dodecyl sulphate, 10 mM sodium dihydrogenphosphate and 12.5 mM sodium borate was found to be the most suitable electrolyte for this separation, whereby the contents of the six flavonoids in crude Scutellariae Radix could easily be determined within about 25 min. The effects of surfactant concentration, pH and temperature on the migration behaviour of the solutes were studied.

引用

页码：221 / 226

页数：6

共 29 条

[1] SEPARATION OF SULFONAMIDES AND DETERMINATION OF THE ACTIVE INGREDIENTS IN TABLETS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY [J].