CHARACTERIZATION OF 2 EPSTEIN-BARR-VIRUS EPITOPES RESTRICTED BY HLA-B7

被引:58
作者
HILL, A
WORTH, A
ELLIOTT, T
ROWLANDJONES, S
BROOKS, J
RICKINSON, A
MCMICHAEL, A
机构
[1] JOHN RADCLIFFE HOSP,INST MOLEC MED,OXFORD OX3 9DU,ENGLAND
[2] UNIV BIRMINGHAM,SCH MED,DEPT CANC STUDIES,CRC LABS,BIRMINGHAM,W MIDLANDS,ENGLAND
关键词
HLA-B7; EPSTEIN-BARR VIRUS; PEPTIDE BINDING;
D O I
10.1002/eji.1830250105
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have identified two epitopes for Epstein-Barr virus specific cytotoxic T lymphocytes (CTL) restricted by the common allele HLA-B7. They are EBNA 3C 881-9 (QPRAPIRPI) and EBNA 3A 379-387 (RPPIFIRRL). The epitopes conform well to the recently described motif for HLA-B7-binding peptides (Huckzo et al., J. Immunol. 1993. 151: 2572). Titration of the peptides in CTL assays and detergent lysate binding assays revealed that extending the peptides at either the N or C terminus did not reduce their affinity for HLA-B7. This behavior contrasted with HLA-B51, which binds peptides with a similar motif to B7, and has identical amino acid residues at sites expected to form the ''F'' pocket of the peptide-binding groove. HLA-B51 also bound the peptide EBNA 3C 881-9, but was unable to bind peptides extended at the C terminus.
引用
收藏
页码:18 / 24
页数:7
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