ISOLATION AND CHARACTERIZATION OF AN OLIVE ALLERGEN-LIKE PROTEIN FROM LILAC POLLEN - SEQUENCE-ANALYSIS OF 3 CDNA-ENCODING PROTEIN ISOFORMS

被引:41
作者
BATANERO, E
VILLALBA, M
LOPEZOTIN, C
RODRIGUEZ, R
机构
[1] UNIV COMPLUTENSE,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-28040 MADRID,SPAIN
[2] UNIV OVIEDO,FAC MED,DEPT BIOL FUNCT,OVIEDO,SPAIN
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 221卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1994.tb18728.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An olive allergen-like protein has been isolated from lilac (Syringa vulgaris) pollen extract. The protein can be considered as an allergen since is recognized by IgE from olive hypersensitive human sera, and has been called Syr v I (IUIS nomenclature). This protein consists of a glycosylated polypeptide of 20 kDa, which has an amino acid composition, spectroscopic properties, and an N-terminal sequence similar to the major allergen from olive pollen, Ole e I. The Lilac allergen is recognized by rabbit polyclonal antisera raised against olive allergen as well as by an Ole e I-specific monoclonal antibody. Using a polymerase chain reaction strategy, based on the similarities observed between these olive and lilac proteins, three cDNA clones encoding Syr v I have been isolated and sequenced. These clones code for a polymorphic protein of 145 residues with a derived molecular mass of about 16400Da, which contains a potential N-glycosylation site. Comparison of the deduced amino acid sequences of these Syr v I isoforms to each other revealed identities of 90-97%. Moreover, these sequences showed a high degree of similarity (85.5-89.6% identity) with Ole e I. The structural and immunological characterization of Syr v I justify the cross-reactions observed between olive and lilac pollen extracts. The molecular cloning of Syr v I is relevant for the epitope mapping in Oleaceae allergens, and may contribute to an improvement in the design of reagents for diagnosis and therapy of IgE-dependent allergic reactions.
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页码:187 / 193
页数:7
相关论文
共 39 条
  • [1] CLINICALLY SIGNIFICANT CROSS-REACTIVITIES AMONG ALLERGENS
    AALBERSE, RC
    [J]. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1992, 99 (2-4) : 261 - 264
  • [2] OLIVE (OLEA-EUROPEA) AND PRIVET (LIGUSTRUM-VULGARE) POLLEN ALLERGENS - IDENTIFICATION AND CROSS-REACTIVITY WITH GRASS-POLLEN PROTEINS
    BALDO, BA
    PANZANI, RC
    BASS, D
    ZERBONI, R
    [J]. MOLECULAR IMMUNOLOGY, 1992, 29 (10) : 1209 - 1218
  • [3] GLYCOSYLATION SITE OF THE MAJOR ALLERGEN FROM OLIVE TREE POLLEN - ALLERGENIC IMPLICATIONS OF THE CARBOHYDRATE MOIETY
    BATANERO, E
    VILLALBA, M
    RODRIGUEZ, R
    [J]. MOLECULAR IMMUNOLOGY, 1994, 31 (01) : 31 - 37
  • [4] ULTRAVIOLET ABSORPTION SPECTRA OF PROTEINS AND AMINO ACIDS
    BEAVEN, GH
    HOLIDAY, ER
    [J]. ADVANCES IN PROTEIN CHEMISTRY, 1952, 7 : 319 - 386
  • [5] ALLERGY IN THE MEDITERRANEAN AREA .3. CROSS-REACTIVITY AMONG OLEACEAE POLLENS
    BOUSQUET, J
    GUERIN, B
    HEWITT, B
    LIM, S
    MICHEL, FB
    [J]. CLINICAL ALLERGY, 1985, 15 (05): : 439 - 448
  • [6] 4 RECOMBINANT ISOFORMS OF COR ALPHA-I, THE MAJOR ALLERGEN OF HAZEL POLLEN, SHOW DIFFERENT IGE-BINDING PROPERTIES
    BREITENEDER, H
    FERREIRA, F
    HOFFMANNSOMMERGRUBER, K
    EBNER, C
    BREITENBACH, M
    RUMPOLD, H
    KRAFT, D
    SCHEINER, O
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 212 (02): : 355 - 362
  • [7] CROSS-REACTIVITY AMONG BIRCH POLLEN, VEGETABLES AND FRUITS AS DETECTED BY IGE ANTIBODIES IS DUE TO AT LEAST 3 DISTINCT CROSS-REACTIVE STRUCTURES
    CALKHOVEN, PG
    AALBERS, M
    KOSHTE, VL
    POS, O
    OEI, HD
    AALBERSE, RC
    [J]. ALLERGY, 1987, 42 (05) : 382 - 390
  • [8] SEQUENCE-ANALYSIS OF CDNA CODING FOR A MAJOR HOUSE DUST MITE ALLERGEN, DER-P-1 HOMOLOGY WITH CYSTEINE PROTEASES
    CHUA, KY
    STEWART, GA
    THOMAS, WR
    SIMPSON, RJ
    DILWORTH, RJ
    PLOZZA, TM
    TURNER, KJ
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (01) : 175 - 182
  • [9] CITY SPORE CONCENTRATIONS IN THE EUROPEAN-ECONOMIC-COMMUNITY (EEC) .7. OLEACEAE (FRAXINUS, LIGUSTRUM, OLEA)
    DAMATO, G
    MULLINS, J
    NOLARD, N
    SPIEKSMA, FTM
    WACHTER, R
    [J]. CLINICAL ALLERGY, 1988, 18 (06): : 541 - 547
  • [10] TREE POLLEN ALLERGY .3. CROSS REACTIONS BASED ON RESULTS FROM SKIN PRICK TESTS AND THE RAST IN HAY-FEVER PATIENTS - A MULTICENTER STUDY
    ERIKSSON, NE
    WIHL, JA
    ARRENDAL, H
    STRANDHEDE, SO
    [J]. ALLERGY, 1987, 42 (03) : 205 - 214