DETERMINATION OF GINSENOSIDES IN GINSENG CRUDE EXTRACTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：48

作者：

CHUANG, WC ^{[1
]}

SHEU, SJ ^{[1
]}

机构：

[1] NATL TAIWAN NORMAL UNIV, DEPT CHEM, TAIPEI, TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 685卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)00724-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the simultaneous determination of ginsenosides Rb-1, Rb-2, Rc, Rd, Re, Rf, Rg(1), Rg2, R(0) and malonylginsenosides Rb-1, Rb-2, and Re was developed. Detection at 198 nm with linear gradient elution with dihydrogenphosphate buffer-far-UV-grade acetonitrile was found to be the most suitable and the contents of the ginsenosides in a non-pretreatment ginseng extract could easily be determined within 45 min. Validation of this method and differences in column selectivity are described.

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页码：243 / 251

页数：9

相关论文

共 14 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF GINSENG SAPONIN BY ULTRAVIOLET DERIVATIZATION [J].

BESSO, H ;

SARUWATARI, Y ;

FUTAMURA, K ;

KUNIHIRO, K ;

FUWA, T ;

TANAKA, O .

PLANTA MEDICA, 1979, 37 (03) :226-233

[2]

HSU HY, 1985, CHINESE MAT MED CONC, P31

[3] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SYSTEM FOR A WIDE-RANGE OF NATURALLY-OCCURRING GLYCOSIDES [J].

KAIZUKA, H ;

TAKAHASHI, K .

JOURNAL OF CHROMATOGRAPHY, 1983, 258 (MAR) :135-146

[4] SIMULTANEOUS DETERMINATION OF GINSENOSIDES AND SAIKOSAPONINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KANAZAWA, H ;

NAGATA, Y ;

MATSUSHIMA, Y ;

TOMODA, M ;

TAKAI, N .

JOURNAL OF CHROMATOGRAPHY, 1990, 507 :327-332

[5] PREPARATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON CHEMICALLY MODIFIED POROUS-GLASS - ISOLATION OF ACIDIC SAPONINS FROM GINSENG [J].

KANAZAWA, H ;

NAGATA, Y ;

MATSUSHIMA, Y ;

TOMODA, M ;

TAKAI, N .

JOURNAL OF CHROMATOGRAPHY, 1991, 537 (1-2) :469-474

[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF GINSENOSIDES IN PANAX-GINSENG EXTRACTS USING GLASS-ODS COLUMN [J].

KANAZAWA, H ;

NAGATA, Y ;

MATSUSHIMA, Y ;

TOMODA, M ;

TAKAI, N .

CHROMATOGRAPHIA, 1987, 24 :517-519

[7]

KITAGAWA I, 1983, CHEM PHARM BULL, V31, P3353

[8] UTILIZING COLUMN SELECTIVITY IN DEVELOPING A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR GINSENOSIDE ASSAY [J].

PETERSEN, TG ;

PALMQVIST, B .

JOURNAL OF CHROMATOGRAPHY, 1990, 504 (01) :139-149

[9] IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF GINSENOSIDES IN PANAX GINSENG EXTRACTS AND PRODUCTS [J].

PIETTA, P ;

MAURI, P ;

RAVA, A .

JOURNAL OF CHROMATOGRAPHY, 1986, 356 (01) :212-219

[10] HPLC SEPARATION AND QUANTITATIVE-DETERMINATION OF GINSENOSIDES FROM PANAX GINSENG, PANAX QUINQUEFOLIUM AND FROM GINSENG DRUG PREPARATIONS .2. [J].

SOLDATI, F ;

STICHER, O .

PLANTA MEDICA, 1980, 39 (04) :348-357