LINKAGE MAPPING OF THE HIGHLY INFORMATIVE DNA MARKER D21S156 TO HUMAN CHROMOSOME-21 USING A POLYMORPHIC GT DINUCLEOTIDE REPEAT

被引：29

作者：

LEWIS, JG

WEBER, JL

PETERSEN, MB

SLAUGENHAUPT, SA

KWITEK, A

MAY, PE

WARREN, AC

CHAKRAVARTI, A

ANTONARAKIS, SE

机构：

[1] MARSHFIELD MED RES FDN,MARSHFIELD,WI 54449

[2] JOHNS HOPKINS UNIV,SCH MED,CTR MED GENET,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT,BALTIMORE,MD 21205

[4] JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT,BALTIMORE,MD 21205

[5] UNIV PITTSBURGH,DEPT HUMAN GENET,PITTSBURGH,PA 15261

[6] UNIV PITTSBURGH,DEPT PSYCHIAT,PITTSBURGH,PA 15261

来源：

GENOMICS | 1990年 / 8卷 / 02期

关键词：

D O I：

10.1016/0888-7543(90)90300-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A (GT)n repeat within the anonymous DNA sequence D21S156 was shown to be highly polymorphic in DNA from members of the 40 CEPH families. At least 12 alleles of this locus were recognized by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction (PCR) using primers flanking the (GT)n repeat. The polymorphism information content was 0.82. PCR amplification of DNA from somatic cell hybrid lines mapped D21S156 to human chromosome 21 and linkage analysis localized this marker close to the loci ETS2, D21S3, and HMG14 on chromosomal band 21q22.3. This polymorphism is highly informative and can serve as anchor locus for human chromosome 21. © 1990.

引用

页码：400 / 402

页数：3

共 18 条

[1] THE POLYDEOXYADENYLATE TRACT OF ALU REPETITIVE ELEMENTS IS POLYMORPHIC IN THE HUMAN GENOME [J].