ANALYSIS OF REPETITIVE DNA-SEQUENCES FROM POTATO CYST NEMATODES AND THEIR USE AS DIAGNOSTIC PROBES

被引：21

作者：

STRATFORD, R

SHIELDS, R

GOLDSBROUGH, AP

FLEMING, C

机构：

[1] DEPT AGR NO IRELAND,NEWTOWNABBEY BT36 7ED,ANTRIM,NORTH IRELAND

[2] QUEENS UNIV BELFAST,NEWTOWNABBEY BT36 7ED,ANTRIM,NORTH IRELAND

来源：

PHYTOPATHOLOGY | 1992年 / 82卷 / 08期

关键词：

D O I：

10.1094/Phyto-82-881

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The potato cyst nematode species Globodera rostochiensis and G. pallida pathotype Pa2/Pa3, as well as G. pallida pathotype Pal, can be distinguished from each other by restriction fragment length polymorphisms using DNA probes, which are present at low frequencies in the nematode genome. By differential screening of nematode DNA we have isolated DNA sequences that are highly abundant in G. rostochiensis, G. pallida, or the G. pallida Pal pathotype. These DNAs can be used as probes to distinguish potato cyst nematodes by a simple dot blot procedure using single cysts. The genomic organization of these highly abundant sequences has been examined; some of them are found to exist in long tandem arrays of 200 copies or more.

引用

页码：881 / 886

页数：6

共 29 条

[11] THE IDENTIFICATION OF THE POTATO CYST NEMATODES GLOBODERA-ROSTOCHIENSIS AND GLOBODERA-PALLIDA BY ISOELECTRIC-FOCUSING OF PROTEINS ON POLYACRYLAMIDE GELS [J].