PROTEIN-KINASE-C ACTIVATORS INHIBIT RECEPTOR-MEDIATED POTOCYTOSIS BY PREVENTING INTERNALIZATION OF CAVEOLAE

被引：172

作者：

SMART, EJ ^{[1
]}

FOSTER, DC ^{[1
]}

YING, YS ^{[1
]}

KAMEN, BA ^{[1
]}

ANDERSON, RGW ^{[1
]}

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT PEDIAT,DALLAS,TX 75235

来源：

JOURNAL OF CELL BIOLOGY | 1994年 / 124卷 / 03期

关键词：

D O I：

10.1083/jcb.124.3.307

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Potocytosis is an endocytic pathway that utilizes glycosylphosphatidylinositol-anchored membrane proteins and caveolae to concentrate and internalize small molecules. We now report that activators of protein kinase C are potent inhibitors of potocytosis. Activators such as phorbol-12-myristate-13-acetate (PMA) inhibit the internalization of receptors for 5-methyltetrahydrofolate but allow the internal receptor pool to return to the cell surface. PMA does not affect the clustering of the folate receptor but instead markedly reduces the number of caveolae. Exposure to PMA totally blocks the intracellular accumulation of 5-methyltetrahydrofolate without affecting receptor-independent uptake or the formation of polyglutamylated species of 5-methyltetrahydrofolate in the cytoplasm. These data suggest that PMA inhibits uptake by inactivating caveolae internalization.

引用

页码：307 / 313

页数：7

共 35 条

[21]

KAMEN BA, 1988, J BIOL CHEM, V263, P13602

[22] THE FOLATE RECEPTOR WORKS IN TANDEM WITH A PROBENECID-SENSITIVE CARRIER IN MA 104 CELLS-INVITRO [J].