ALTERATIONS IN THE SEQUENCE OF THE GENE-4 FROM A HUMAN ROTAVIRUS AFTER MULTIPLE PASSAGES IN HEPG2 LIVER-CELLS

被引：12

作者：

KITAMOTO, N ^{[1
]}

MATTION, NM ^{[1
]}

ESTES, MK ^{[1
]}

机构：

[1] BAYLOR COLL MED,DIV MOLEC VIROL,1 BAYLOR PLAZA,HOUSTON,TX 77030

来源：

ARCHIVES OF VIROLOGY | 1993年 / 130卷 / 1-2期

关键词：

D O I：

10.1007/BF01319006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The human Wa strain of rotaviruses, initially unable to grow in liver cells, was adapted by multiple passages to grow in HepG2 cells. The genome segment 4 of both the parental and passaged strains was cloned and sequenced. Five amino acid differences (residues 38, 120, 421, 525, and 618) were found in the HepG2-passaged variant compared to the parental Wa strain. Our results support the hypothesis that viral variants that have improved capabilities for infecting liver cells can be generated during infection.

引用

页码：179 / 185

页数：7

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