NA,K-ATPASE BETA-SUBUNIT ISOFORM EXPRESSION IN THE PERIPHERAL NERVOUS-SYSTEM OF THE RAT

被引：16

作者：

FINK, DJ ^{[1
]}

FANG, DN ^{[1
]}

LI, TD ^{[1
]}

MATA, M ^{[1
]}

机构：

[1] UNIV MICHIGAN, DEPT NEUROL, ANN ARBOR, MI USA

来源：

NEUROSCIENCE LETTERS | 1995年 / 183卷 / 03期

关键词：

SODIUM POTASSIUM TRANSPORTING ATPASE; AXONAL TRANSPORT; DORSAL ROOT GANGLION; SCHWANN CELL; SCIATIC NERVE;

D O I：

10.1016/0304-3940(94)11152-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Using the RNase protection assay (RPA) to study the distribution of isoforms of the non-catalytic (beta) subunit of Na,K-ATPase in the peripheral nervous system, we found both beta 1 and beta 2 isoform mRNAs in dorsal root ganglion (DRG), but only beta 2 mRNA in sciatic nerve. Using Western blot to measure accumulation of the polypeptides at a ligature on the nerve we found that beta 1 but not beta 2 polypeptide is carried by rapid axonal transport in the sciatic nerve. These results imply that beta 1 is the prominent isoform of Na,K-ATPase in neurons and beta 2 the prominent isoform in Schwann cells.

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收藏

页码：206 / 209

页数：4

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