PREPARATIVE FLATBED ELECTROFOCUSING IN GRANULATED GELS WITH NATURAL PH GRADIENTS GENERATED FROM SIMPLE BUFFERS

被引：51

作者：

PRESTIDGE, RL ^{[1
]}

HEARN, MTW ^{[1
]}

机构：

[1] UNIV OTAGO, SCH MED, MED RES COUNCIL NEW ZEALAND, IMMUNOPATHOL RES UNIT, DUNEDIN, NEW ZEALAND

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0003-2697(79)90332-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Techniques are reported for preparative electrofocusing in natural pH gradients formed from simple buffers using flatbeds of granulated gels. The stability and reproducibility of the gradients permit excellent resolution of complex mixtures of proteins. Examples reported in this paper include the preparative separation and recovery of biologically active serum and thyroid proteins. © 1979.

引用

页码：95 / 102

页数：8

共 32 条

[1] QUANTITATIVE REMOVAL OF CARRIER AMPHOLYTES FROM PROTEIN-FRACTIONS DERIVED FROM ISOELECTRIC FOCUSING [J].

BAUMANN, G ;

CHRAMBACH, A .

ANALYTICAL BIOCHEMISTRY, 1975, 69 (02) :649-651

[2] LACK OF INTERACTION BETWEEN HORMONAL PROTEINS AND SYNTHETIC CARRIER AMPHOLYTES (AMPHOLINE) [J].

BAUMANN, G ;

CHRAMBACH, A .

ANALYTICAL BIOCHEMISTRY, 1975, 64 (02) :530-536

[3] POLYACRYLAMIDE-GEL ISOELECTRIC FOCUSING OF PROTEINS - DETERMINATION OF ISOELECTRIC POINTS USING AN ANTIMONY ELECTRODE [J].

BEELEY, JA ;

STEVENSON, SM ;

BEELEY, JG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 285 (02) :293-300

[4] ISOELECTRIC-FOCUSING OF INTERACTING SYSTEMS .1. CARRIER AMPHOLYTE-INDUCED MACROMOLECULAR ISOMERIZATION [J].

CANN, JR ;

STIMPSON, DI .

BIOPHYSICAL CHEMISTRY, 1977, 7 (02) :103-114

[5] NATURAL PH GRADIENTS FORMED BY AMINO-ACIDS - AMPHOLYTE DISTRIBUTION, TIME COURSE, USE IN ELECTROFOCUSING OF PROTEIN, RELATION TO PH GRADIENTS IN ISOTACHOPHORESIS, AND SEPARATOR EFFECTS [J].

CASPERS, ML ;

CHRAMBACH, A .

ANALYTICAL BIOCHEMISTRY, 1977, 81 (01) :28-39

[6] DEVICE FOR AUTOMATED MEASUREMENT OF PH GRADIENTS IN GEL ELECTROFOCUSING [J].

CHIDAKEL, BE ;

NGUYEN, NY ;

CHRAMBACH, A .

ANALYTICAL BIOCHEMISTRY, 1977, 77 (01) :216-225

[7]

FELGENHAUER K, 1973, Annals of the New York Academy of Sciences, V209, P147

[8]

FELGENHAUER K, 1971, PROTIDES BIOL FLUIDS, V19, P575

[9] ARTIFACTS IN ISOELECTRIC FOCUSING [J].

FRATER, R .

JOURNAL OF CHROMATOGRAPHY, 1970, 50 (03) :469-&

[10]

FUKUDA M, 1969, TUMOR RES, V4, P57

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