PREPARATIVE FLATBED ELECTROFOCUSING IN GRANULATED GELS WITH NATURAL PH GRADIENTS GENERATED FROM SIMPLE BUFFERS

被引：51

作者：

PRESTIDGE, RL ^{[1
]}

HEARN, MTW ^{[1
]}

机构：

[1] UNIV OTAGO, SCH MED, MED RES COUNCIL NEW ZEALAND, IMMUNOPATHOL RES UNIT, DUNEDIN, NEW ZEALAND

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0003-2697(79)90332-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Techniques are reported for preparative electrofocusing in natural pH gradients formed from simple buffers using flatbeds of granulated gels. The stability and reproducibility of the gradients permit excellent resolution of complex mixtures of proteins. Examples reported in this paper include the preparative separation and recovery of biologically active serum and thyroid proteins. © 1979.

引用

页码：95 / 102

页数：8