DETECTION OF HERPES-SIMPLEX VIRUS-DNA IN HUMAN TEAR FILM BY THE POLYMERASE CHAIN-REACTION

被引：54

作者：

YAMAMOTO, S

SHIMOMURA, Y

KINOSHITA, S

NISHIDA, K

YAMAMOTO, R

TANO, Y

机构：

[1] KYOTO PREFECTURAL UNIV MED,DEPT OPHTHALMOL,KYOTO,JAPAN

[2] KANSAI MED UNIV,DEPT OPHTHALMOL,OSAKA,OSAKA,JAPAN

来源：

AMERICAN JOURNAL OF OPHTHALMOLOGY | 1994年 / 117卷 / 02期

关键词：

D O I：

10.1016/S0002-9394(14)73071-5

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

We investigated the use of the polymerase chain reaction for detecting genomes of herpes simplex virus, varicella-zoster virus, and cytomegalovirus from tear film of patients with clinically diagnosed herpes simplex virus keratitis. Using the polymerase chain reaction with a herpes simplex virus detection sensitivity adjusted to 1.0 plaque-forming units/ml, we detected herpes simplex virus genomic sequences in 12 of 12 epithelial keratitis specimens, two of six stromal keratitis specimens, but in none of 20 normal specimens. Neither varicella-zoster virus nor cytomegalovirus genomic sequences were detected in any sample. These results suggest that polymerase chain reaction quickly performed with reduced sensitivity is useful as a diagnostic tool for confirming clinical observations.

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页码：160 / 163

页数：4

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