ESSENTIAL ARGINYL RESIDUE IN YEAST HEXOKINASE

被引:44
作者
PHILIPS, M [1 ]
PHO, DB [1 ]
PRADEL, LA [1 ]
机构
[1] COLL FRANCE, BIOCHIM CELLULAIRE LAB, F-75005 PARIS, FRANCE
关键词
(Yeast); Arginyl residue; Hexokinase;
D O I
10.1016/0005-2744(79)90033-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The inactivation of yeast hexokinase A (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) by phenylglyoxal obeys pseudo 1st-order kinetics. Formation of a reversible enzyme-reagent complex prior to modification is suggested by the observed saturation kinetics. Loss of activity correlates with the incorporation of 1 mol of [14C]phenylglyoxal/mol 50,000 dalton subunit. No significant conformational change occurs concomitantly. Inactivation is attributable to modification of an arginyl residue. The pattern of protection by substrates and analogs favors an interaction of this essential resude with the terminal phosphoryl group of ATP or G-6-P.
引用
收藏
页码:296 / 304
页数:9
相关论文
共 32 条
  • [11] KITZ R, 1962, J BIOL CHEM, V237, P3245
  • [12] YEAST HEXOKINASE .I. PREPARATION OF PURE ENZYME
    LAZARUS, NR
    RAMEL, AH
    RUSTUM, YM
    BARNARD, EA
    [J]. BIOCHEMISTRY, 1966, 5 (12) : 4003 - &
  • [13] LEVY HM, 1963, J BIOL CHEM, V238, P3654
  • [14] FUNCTIONAL ARGININE RESIDUE IN RABBIT-MUSCLE ALDOLASE
    LOBB, RR
    STOKES, AM
    HILL, HAO
    RIORDAN, JF
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 70 (02): : 517 - 522
  • [15] STUDIES ON ACTIVE-SITE OF YEAST HEXOKINASE - SPECIFIC PHOSPHORYLATION OF A SERINE RESIDUE INDUCED BY D-XYLOSE AND ATPMG
    MENEZES, LC
    PUDLES, J
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 65 (01): : 41 - 47
  • [16] SPECIFIC PHOSPHORYLATION OF YEAST HEXOKINASE INDUCED BY XYLOSE AND ATPMG - PROPERTIES OF PHOSPHORYLATED FORM OF ENZYME
    MENEZES, LC
    PUDLES, J
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 178 (01) : 34 - 42
  • [17] EVIDENCE FOR A SINGLE ESSENTIAL THIOL IN YEAST HEXOKINASE MOLECULE
    OTIENO, S
    BHARGAVA, AK
    SERELIS, D
    BARNARD, EA
    [J]. BIOCHEMISTRY, 1977, 16 (19) : 4249 - 4255
  • [18] YEAST 3-PHOSPHOGLYCERATE KINASE - ESSENTIAL ARGINYL RESIDUES AT 3-PHOSPHOGLYCERATE BINDING-SITE
    PHILIPS, M
    ROUSTAN, C
    FATTOUM, A
    PRADEL, LA
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 523 (02) : 368 - 376
  • [19] PHO DB, 1977, BIOCHEMISTRY-US, V16, P4533
  • [20] PHO DB, 1977, H-S Z PHYSIOL CHEM, V358, P1258