EFFECT OF HYDRALAZINE AND DIHYDRALAZINE ON CONNECTIVE-TISSUE AND BINDING TO SERUM-PROTEIN

Hydralazine and dihydralazine, chelators of Fe2+, Fe3+ and Mn2+ions, inhibited collagen biosynthesis in organ culture of chicken embryo tibiae. Both substances inhibited the in vitro hydroxylation of [14C]Pro-labelled protocollagen by protocollagen proline hydroxylase. A decreased incorporation of [14C]-D-glucosamine into glyco-and/or muco-proteins was found under the effect of the two drugs. Dihydralazine, a cationic compound, was bound to DNA and acid mucopolysaccharides (glycosaminoglycans), forming an insoluble complex. Both substances were bound to serum arglobulin. Subcutaneous injections of dihydralazine in rats produced a local as well as a general toxic effect. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.