CLONING OF THE 2 PYRUVATE-KINASE ISOENZYME STRUCTURAL GENES FROM ESCHERICHIA-COLI - THE RELATIVE ROLES OF THESE ENZYMES IN PYRUVATE BIOSYNTHESIS

被引：96

作者：

PONCE, E ^{[1
]}

FLORES, N ^{[1
]}

MARTINEZ, A ^{[1
]}

VALLE, F ^{[1
]}

BOLIVAR, F ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, INST BIOTECNOL, DEPT MOLEC MICROBIOL, CUERNAVACA 62271, MORELOS, MEXICO

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 19期

关键词：

D O I：

10.1128/jb.177.19.5719-5722.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism. These genes were insertionally inactivated with antibiotic resistance markers and utilized to interrupt one or both pyk genes in the E. coli chromosome. With these constructions, we were able to study the role of these isoenzymes in pyruvate biosynthesis.

引用

页码：5719 / 5722

页数：4

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