EXPRESSION OF PROTEIN-KINASE-C ISOZYMES IN RAT GLIAL-CELL LINE

被引：2

作者：

KUMAR, K ^{[1
]}

KIM, B ^{[1
]}

RUPP, H ^{[1
]}

MADHUKAR, B ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT PEDIAT & HUMAN DEV,E LANSING,MI 48824

来源：

NEUROREPORT | 1993年 / 4卷 / 04期

关键词：

PROTEIN KINASE-C; ISOZYMES; GLIA; IMMUNOFLUORESCENCE;

D O I：

10.1097/00001756-199304000-00023

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To study the presence and distribution of various isozymes of PKC in glial cells, immunofluorescent studies were performed on a cortical glial cell line culture derived from rat cerebral cortex and analyzed by a highly sensitive automated laser cytometric device, ACAS-570 (Adherent Cell Analysis and Sorting). The antibodies tested were against alpha, beta, gamma, and epsilon isozymes of PKC, and the catalytic domain (cd), i.e. antibody against the cd to all isozymes of PKC. The data indicate that rat cortical glial cell line expresses at least three isozymes of PKC, epsilon, beta, and alpha, the degree of intensity of immunofluorescence being epsilon > beta > alpha. The immunofluorescence for PKC-cd was the most intense.

引用

页码：431 / 434

页数：4

共 26 条

[1] DISTINCT PATTERNS OF EXPRESSION OF DIFFERENT PROTEIN-KINASE-C MESSENGER-RNAS IN RAT-TISSUES
BRANDT, SJ
NIEDEL, JE
BELL, RM
YOUNG, WS
[J]. CELL, 1987, 49 (01) : 57 - 63
[2] DECREASED LEVELS OF PROTEIN KINASE-C IN ALZHEIMER BRAIN
COLE, G
DOBKINS, KR
HANSEN, LA
TERRY, RD
SAITOH, T
[J]. BRAIN RESEARCH, 1988, 452 (1-2) : 165 - 174
[3] MULTIPLE, DISTINCT FORMS OF BOVINE AND HUMAN PROTEIN-KINASE-C SUGGEST DIVERSITY IN CELLULAR SIGNALING PATHWAYS
COUSSENS, L
PARKER, PJ
RHEE, L
YANGFENG, TL
CHEN, E
WATERFIELD, MD
FRANCKE, U
ULLRICH, A
[J]. SCIENCE, 1986, 233 (4766) : 859 - 866
[4] HAUNG FL, 1987, BIOCHEM BIOPH RES CO, V149, P946
[5] PURIFICATION AND CHARACTERIZATION OF 3 TYPES OF PROTEIN-KINASE-C FROM RABBIT BRAIN CYTOSOL
JAKEN, S
KILEY, SC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (13) : 4418 - 4422
[6] THE PROTEIN KINASE-C FAMILY - HETEROGENEITY AND ITS IMPLICATIONS
KIKKAWA, U
KISHIMOTO, A
NISHIZUKA, Y
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 : 31 - 44
[7] CLONING AND EXPRESSION OF MULTIPLE PROTEIN-KINASE-C CDNAS
KNOPF, JL
LEE, MH
SULTZMAN, LA
KRIZ, RW
LOOMIS, CR
HEWICK, RM
BELL, RM
[J]. CELL, 1986, 46 (04) : 491 - 502
[8] MASLIAH E, 1990, J NEUROSCI, V10, P2113
[9] DIFFERENTIAL EXPRESSION OF PROTEIN-KINASE-C ISOZYMES IN RAT GLIAL-CELL CULTURES
MASLIAH, E
YOSHIDA, K
SHIMOHAMA, S
GAGE, FH
SAITOH, T
[J]. BRAIN RESEARCH, 1991, 549 (01) : 106 - 111
[10] MOCHLYROSEN D, 1987, J BIOL CHEM, V262, P2291

← 1 2 3 →