EXPRESSION OF PROTEIN-KINASE-C ISOZYMES IN RAT GLIAL-CELL LINE

To study the presence and distribution of various isozymes of PKC in glial cells, immunofluorescent studies were performed on a cortical glial cell line culture derived from rat cerebral cortex and analyzed by a highly sensitive automated laser cytometric device, ACAS-570 (Adherent Cell Analysis and Sorting). The antibodies tested were against alpha, beta, gamma, and epsilon isozymes of PKC, and the catalytic domain (cd), i.e. antibody against the cd to all isozymes of PKC. The data indicate that rat cortical glial cell line expresses at least three isozymes of PKC, epsilon, beta, and alpha, the degree of intensity of immunofluorescence being epsilon > beta > alpha. The immunofluorescence for PKC-cd was the most intense.