ONLINE CULTURE FLUORESCENCE MEASUREMENT DURING THE BATCH CULTIVATION OF POLY-BETA-HYDROXYBUTYRATE PRODUCING ALCALIGENES-EUTROPHUS

被引：14

作者：

GROOM, CA ^{[1
]}

LUONG, JHT ^{[1
]}

MULCHANDANI, A ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

JOURNAL OF BIOTECHNOLOGY | 1988年 / 8卷 / 04期

关键词：

Biopolymers - Biotechnology - Cell Culture - Fluorescence--Measurements - Microorganisms;

D O I：

10.1016/0168-1656(88)90019-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Estimation of biomass concentration by on-line fluorimetry was examined during batch cultivation is of Alcaligenes eutrophus TC 17697, a poly-β-hydroxybutyric acid (PHB) producer. The results obtained revealed a linear correlation between total culture fluorescence and total biomass concentration. The total fluorescence level was attributed to cellular fluorescence and to the fluoresence of compounds presumably produced by the cells and secreted into the culture medium. An increase in the specific cellular fluorescence during the course of the experiments indicated a shift in metabolism that favored the production of PHB.

引用

页码：271 / 278

页数：8

共 20 条

[1] THE INTERPRETATION OF ONLINE PROCESS MEASUREMENTS OF INTRACELLULAR NADH IN FERMENTATION PROCESSES
ARMIGER, WB
FORRO, JF
MONTALVO, LM
LEE, JF
ZABRISKIE, DW
[J]. CHEMICAL ENGINEERING COMMUNICATIONS, 1986, 45 (1-6) : 197 - 206
[2] ONLINE MEASUREMENTS OF CULTURE FLUORESCENCE - METHOD AND APPLICATION
BEYELER, W
EINSELE, A
FIECHTER, A
[J]. EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1981, 13 (01): : 10 - 14
[3] RAPID GAS-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF POLY-BETA-HYDROXYBUTYRIC ACID IN MICROBIAL BIOMASS
BRAUNEGG, G
SONNLEITNER, B
LAFFERTY, RM
[J]. EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 6 (01): : 29 - 37
[4] POLYMER SYNTHESIS BY MICROORGANISMS - TECHNOLOGY AND ECONOMICS
BYROM, D
[J]. TRENDS IN BIOTECHNOLOGY, 1987, 5 (09) : 246 - 250
[5] TAXONOMIC STUDIES ON SOME GRAM NEGATIVE POLARLY FLAGELLATED HYDROGEN-BACTERIA AND RELATED SPECIES
DAVIS, DH
STANIER, RY
DOUDOROF.M
MANDEL, M
[J]. ARCHIV FUR MIKROBIOLOGIE, 1970, 70 (01): : 1 - &
[6] SUBSTRATE UPTAKE MECHANISMS FOR YEAST-CELLS - NEW APPROACH UTILIZING A FLUOROMETER
EINSELE, A
RISTROPH, DL
HUMPHREY, AE
[J]. EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1979, 6 (04): : 335 - 339
[7] HANSON R, 1981, MANUAL METHODS GENER, P358
[8] USE OF ENZYMES FOR THE DETERMINATION OF ENVIRONMENTAL-POLLUTANTS
HO, MH
[J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1987, 31 (01) : 1 - 9
[9] PROCESS-CONTROL DURING THE SUSPENSION-CULTURE OF A HUMAN-MELANOMA CELL-LINE IN A MECHANICALLY STIRRED LOOP BIOREACTOR
LEIST, C
MEYER, HP
FIECHTER, A
[J]. JOURNAL OF BIOTECHNOLOGY, 1986, 4 (04) : 235 - 246
[10] LUONG JHT, 1986, APPL MICROBIOL BIOT, V24, P65

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