USE OF RESORUFIN-LABELED N-GLYCOPEPTIDE IN A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY TO MONITOR ENDOGLYCOSIDASE ACTIVITIES DURING CULTIVATION OF FLAVOBACTERIUM-MENINGOSEPTICUM

被引:11
作者
BOURGERIE, S [1 ]
KARAMANOS, Y [1 ]
BERGER, S [1 ]
JULIEN, R [1 ]
机构
[1] UNIV LIMOGES,INST BIOTECHNOL,123 AVE ALBERT THOMAS,F-87060 LIMOGES 5,FRANCE
关键词
ENDOGLYCOSIDASES ASSAY; PNGASE-F; FLUORESCENCE-HPLC;
D O I
10.1007/BF00731160
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase F (PNGase F) and endo-beta-N-acetyl glucosaminidase F (Endo F) activities were monitored during cultivation of Flavobacterium meningosepticum using a new fluorescence-HPLC procedure based on a commercially available substrate. The PNGase F activity reached a maximum level at the end of the log phase and remained constant during the stationary phase, while Endo F continuously increased until late stationary phase. PNGase F obtained at the end of the log phase was less contaminated by other proteins compared with late stationary phase.
引用
收藏
页码:162 / 167
页数:6
相关论文
共 17 条