USE OF RESORUFIN-LABELED N-GLYCOPEPTIDE IN A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY TO MONITOR ENDOGLYCOSIDASE ACTIVITIES DURING CULTIVATION OF FLAVOBACTERIUM-MENINGOSEPTICUM

被引：11

作者：

BOURGERIE, S ^{[1
]}

KARAMANOS, Y ^{[1
]}

BERGER, S ^{[1
]}

JULIEN, R ^{[1
]}

机构：

[1] UNIV LIMOGES,INST BIOTECHNOL,123 AVE ALBERT THOMAS,F-87060 LIMOGES 5,FRANCE

来源：

GLYCOCONJUGATE JOURNAL | 1992年 / 9卷 / 04期

关键词：

ENDOGLYCOSIDASES ASSAY; PNGASE-F; FLUORESCENCE-HPLC;

D O I：

10.1007/BF00731160

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase F (PNGase F) and endo-beta-N-acetyl glucosaminidase F (Endo F) activities were monitored during cultivation of Flavobacterium meningosepticum using a new fluorescence-HPLC procedure based on a commercially available substrate. The PNGase F activity reached a maximum level at the end of the log phase and remained constant during the stationary phase, while Endo F continuously increased until late stationary phase. PNGase F obtained at the end of the log phase was less contaminated by other proteins compared with late stationary phase.

引用

页码：162 / 167

页数：6

共 17 条

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