BA-2+ CURRENT OSCILLATIONS MODULATED BY CYCLIC-AMP AND PHORBOL ESTERS IN RAS-TRANSFORMED FIBROBLASTS

被引：3

作者：

HIGASHIDA, H ^{[1
]}

HOSHI, N ^{[1
]}

NODA, M ^{[1
]}

SHAHIDULLAH, M ^{[1
]}

HASHII, M ^{[1
]}

NOZAWA, Y ^{[1
]}

机构：

[1] GIFU UNIV,SCH MED,DEPT BIOCHEM,GIFU 500,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91864-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An oscillatory influx of divalent cations was measured as Ba2+ inward currents (Ba2+ current oscillations) by voltage-clamp recording in v-Ki-ras-transformed NIH 3T3 (DT) fibroblasts after activation with bradykinin or serum. Application of forskolin or dibutyryl cyclic AMP onto DT cells initiated Ba2+ current oscillations. Increasing intracellular cyclic AMP reduced the amplitude but increased the frequency of the Ba2+ current oscillations. Activation of protein kinase C by phorbol esters terminated Ba2+ current oscillations. No inhibition of Ba2+ current oscillations by phorbol esters was observed in down-regulated cells that had been pretreated with phorbol esters for 24 hrs. The results suggest that Ba2+ current oscillations are regulated by intracellular second messengers. © 1992.

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页码：1240 / 1245

页数：6

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