LINKAGES AMONG RFLP, RAPD, ISOZYME, DISEASE RESISTANCE, AND MORPHOLOGICAL MARKERS IN NARROW AND WIDE CROSSES OF CUCUMBER

被引:134
作者
KENNARD, WC [1 ]
POETTER, K [1 ]
DIJKHUIZEN, A [1 ]
MEGLIC, V [1 ]
STAUB, JE [1 ]
HAVEY, MJ [1 ]
机构
[1] UNIV WISCONSIN,DEPT HORT,USDA ARS,VEGETABLE CROPS UNIT,MADISON,WI 53706
关键词
CUCUMIS SATIVUS; CUCUMIS-SATIVUS VAR HARDWICKII; GENETIC MAP;
D O I
10.1007/BF00226980
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A 58-point genetic map was constructed with RFLP, RAPD, isozyme, morphological, and disease-resistance markers spanning 766 cM on ten linkage groups for a cross within the cultivated cucumber (Cucumis sativus var. sativus). Relatively few DNA polymorphisms were detected, agreeing with previous studies documenting a narrow genetic base for cucumber. Most RFLPs within the cultivated cucumber appear to be changes at restriction-enzyme sites. Sixty-four percent of RAPD markers that fit expected ratios at P<0.001 were unlinked, possibly due to poor amplification and the inefficiency of dominant markers to detect linkage in an F-2 family. A 70-point linkage map, spanning 480 cM on ten linkage groups, was constructed with RFLP, isozyme, morphological, and disease-resistance markers for a cross between the cultivated cucumber and the wild or feral C. sativus var. hardwickii. Unlinked markers and more linkage groups than chromosome pairs indicated that both maps were not saturated. Twenty-one markers doubly segregated in both families and regions of colinearity were identified.
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页码:42 / 48
页数:7
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