IDENTIFICATION AND INITIAL CHARACTERIZATION OF GLUCOSE-REPRESSIBLE PROMOTERS OF STREPTOCOCCUS-MUTANS

被引：12

作者：

LANE, MA

BAYLES, KW

YASBIN, RE

机构：

[1] UNIV MARYLAND,DEPT BIOL SCI,CATONSVILLE,MD 21228

[2] UNIV MARYLAND,PROGRAM MOLEC & CELL BIOL,CATONSVILLE,MD 21228

来源：

GENE | 1991年 / 100卷

关键词：

RECOMBINANT DNA; TRANSCRIPTIONAL FUSIONS; CATABOLITE REPRESSION; DENTAL CARIES;

D O I：

10.1016/0378-1119(91)90371-H

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Three catabolite-repressible promoters from Streptococcus mutans have been isolated. These promoters were identified by utilizing the vector pRQ200 which contains a promoterless amylase-encoding gene, a Gram- origin of replication, and an erythromycin-resistance determinant. A library of S. mutans DNA was constructed in pRQ200, amplified in Escherichia coli and integrated by Campbell-type insertion into the S. mutans chromosome following transformation. Colonies exhibiting amylase production on media lacking an extraneous carbohydrate source were screened for diminished amylase production on media containing glucose. The effect of glucose on these promoters has been characterized using a quantitative spectrophotometric assay of amylase activity.

引用

页码：225 / 229

页数：5

共 24 条

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