ASPARTAME PRECURSOR SYNTHESIS IN WATER MISCIBLE COSOLVENT CATALYZED BY THERMOLYSIN

被引：7

作者：

LEE, KH

LEE, PM

SIAW, YS

MORIHARA, K

机构：

[1] INDIANA UNIV,DEPT CHEM,SHAH ALAM CAMPUS MALAYSIA,ITM MUCIA,SECTION 17,SHAH ALAM 40000,MALAYSIA

[2] UNIV PERTANIAN MALAYSIA,FAC FOOD SCI & BIOTECHNOL,DEPT BIOTECHNOL,SERDANG 43400,MALAYSIA

[3] UNIV E ASIA,INST APPL LIFE SCI,YAMAGUCHI 751,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 12期

关键词：

D O I：

10.1007/BF01027021

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 muM in 25 % methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4-degrees-C and 37-degrees-C were 50 % and 25 % respectively where an increase in peptide yield of 85 % was obtained for both conditions as compared to that in water.

引用

页码：1159 / 1164

页数：6

共 11 条

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