RAPID DETECTION OF FECAL-COLIFORMS IN SEWAGE USING A COLORIMETRIC ASSAY OF BETA-D-GALACTOSIDASE

被引：20

作者：

APTE, SC

DAVIES, CM

PETERSON, SM

机构：

[1] Centre for Advanced Analytical Chemistry, CSIRO Division of Coal and Energy Technology, Menai, NSW 2234

来源：

WATER RESEARCH | 1995年 / 29卷 / 07期

关键词：

BACTERIA; COLORIMETRY; BETA-D-GALACTOSIDASE; FECAL COLIFORMS; SEWAGE; SPECTROPHOTOMETRY; WASTE-WATER; WASTE-WATER TREATMENT;

D O I：

10.1016/0043-1354(95)00010-I

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A rapid colorimetric method has been developed for the detection of faecal coliforms in sewage. The method is based on the hydrolysis of o-nitrophenyl-beta-D-galactopyranoside by the bacterial enzyme beta-D-galactosidase and detection of liberated o-nitrophenol. Enzyme activity correlated well with numbers of faecal coliforms determined by conventional membrane filtration over a range of bacterial concentrations. A lower limit of 10(3) faecal coliforms per mi could be detected within 60 min. It is proposed that the method, which is amenable to automation, may be used asa surrogate for monitoring faecal coliform concentrations in sewage and a variety of effluents with a view to providing an early-warning indicator of sewage contamination or leakage.

引用

页码：1803 / 1806

页数：4