STRUCTURAL STUDIES OF THE SACCHARIDE PART OF THE CELL-ENVELOPE LIPOPOLYSACCHARIDE FROM HAEMOPHILUS-INFLUENZAE STRAIN AH1-3(LIC3+)

被引：65

作者：

SCHWEDA, EKH ^{[1
]}

HEGEDUS, OE ^{[1
]}

BORRELLI, S ^{[1
]}

LINDBERG, AA ^{[1
]}

WEISER, JN ^{[1
]}

MASKELL, DJ ^{[1
]}

MOXON, ER ^{[1
]}

机构：

[1] JOHN RADCLIFFE HOSP,INST MOLEC MED,OXFORD OX3 9DU,ENGLAND

来源：

CARBOHYDRATE RESEARCH | 1993年 / 246卷

关键词：

D O I：

10.1016/0008-6215(93)84043-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the saccharide part of the lipopolysaccharide from Haemophilus influenzae strain AH1-3 (lic3+) has been investigated. The saccharide was obtained from the lipopolysaccharide by mild acid hydrolysis followed by high-performance anion-exchange chromatography, and isolated fractions were studied by methylation analysis, NMR spectroscopy, and FAB mass spectrometry. The major saccharide is a heptasaccharide with the following structure, [GRAPHICS] in which Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid and PEA is 2-aminoethyl phosphate. Hep is identified as L-glycero-D-manno-heptose. The absolute configuration of the phosphorylated heptose is tentative only.

引用

页码：319 / 330

页数：12

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