FLUORESCENCE OF TYROSINE AND TRYPTOPHAN IN PROTEINS USING ONE-PHOTON AND 2-PHOTON EXCITATION

被引：50

作者：

KIERDASZUK, B

GRYCZYNSKI, I

MODRAKWOJCIK, A

BZOWSKA, A

SHUGAR, D

LAKOWICZ, JR

机构：

[1] UNIV MARYLAND,SCH MED,CTR FLUORESCENCE SPECT,DEPT BIOL CHEM,BALTIMORE,MD 21201

[2] UNIV WARSAW,DEPT BIOPHYS,PL-02089 WARSAW,POLAND

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1995年 / 61卷 / 04期

关键词：

D O I：

10.1111/j.1751-1097.1995.tb08615.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the emission spectra of tyrosine- and tryptophan-containing proteins using one-photon (270-310 nm) and two-photon (565-610 nm) excitation. Emission spectra for two-photon excitation of native and denatured human serum albumin and of three purine nucleoside phosphorylases indicated an absence of the tyrosine emission normally seen for one-photon excitation below 290 nm. We examined the one-photon and two-photon excitation spectra of tyrosine-tryptophan mixtures to determine the origin of selective excitation of the tryptophan residues. These results confirmed a short-wavelength shift of the tyrosine two-photon excitation spectrum relative to that of tryptophan, as recently reported by Rehms and Callis (1993) Chem. Phys. Lett. 208, 276-282.

引用

页码：319 / 324

页数：6