IMAGING OF L-TYPE CA2+ CHANNELS IN OLFACTORY-BULB NEURONS USING FLUORESCENT DIHYDROPYRIDINE AND A STYRYL DYE

被引：19

作者：

SCHILD, D

GEILING, H

BISCHOFBERGER, J

机构：

[1] Physiologisches Institut, Universität Göttingen, D-37073 Göttingen

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1995年 / 59卷 / 02期

关键词：

CALCIUM CHANNEL; DIHYDROPYRIDINE; LASER SCANNING MICROSCOPY;

D O I：

10.1016/0165-0270(94)00181-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have imaged the fluorescence of dihydropyridine-Bodipy (fDHP) in cultured olfactory bulb neurones in order to investigate the subcellular distribution of L-type calcium channels in these neurones. The neurones were stained with both fDHP and the voltage-sensitive styryl dye RH414. The fluorescence emission maxima of these dyes were in the green and red ranges of the spectrum, respectively, and were recorded by the 2 photomultiplier channels of a laser scanning microscope. The fDHP images were ratioed with the RH414 images taken simultaneously. The resulting ratio images revealed the spatial distribution of the surface density of L-type calcium channels. This density was highest on somata, in particular at the base of dendrites, and decreased with the distance from this maximum. Two classes of dendritic L-type channel distributions were observed: one with a homogeneously low density and another one with a characteristic gradient of the L-type channel density along proximal dendrites. This subcellular localization of L-type calcium channels is discussed in the light of specific functional roles.

引用

页码：183 / 190

页数：8

共 36 条

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