CALCIFICATION INHIBITORS IN HUMAN LIGAMENTUM-FLAVUM

被引：8

作者：

MARUTA, K ^{[1
]}

ICHIMURA, K ^{[1
]}

MATSUI, H ^{[1
]}

YAMAGAMI, T ^{[1
]}

SANO, A ^{[1
]}

TSUJI, H ^{[1
]}

机构：

[1] TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT ORTHOPAED SURG,TOYAMA,TOYAMA 93001,JAPAN

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 1993年 / 11卷 / 01期

关键词：

D O I：

10.1002/jor.1100110111

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

To examine the presence of substances which inhibit calcification in human ligamentum flavum, the inhibitory effect of an Na2HPO4 extract of the flavum was determined in terms of the in vitro calcium uptake of the ligamentum flavum matrix. Additionally, grafts of extracted and non-extracted dry ligamentum flavum matrices were transplanted into the dorsal muscles of rats, and calcification in the grafts was examined radiologically and histochemically. In order to determine if component cells of human ligamentum flavum produce calcification inhibitors, ligamentum flavum cells were cultured, and the crystal inhibitor activity of the culture medium was measured by a seed test which used hydroxyapatite as the nucleus of precipitation. The calcification reaction system demonstrated that the ligamentum flavum extract contains an inhibitory factor for calcium uptake by the ligamentum flavum matrix. The seed test revealed that human ligamentum flavum cells produce calcification inhibitor activity.

引用

页码：92 / 103

页数：12