INTRACELLULAR CA2+ SIGNALS ACTIVATE APOPTOSIS IN THYMOCYTES - STUDIES USING THE CA2+-ATPASE INHIBITOR THAPSIGARGIN

被引：248

作者：

JIANG, S ^{[1
]}

CHOW, SC ^{[1
]}

NICOTERA, P ^{[1
]}

ORRENIUS, S ^{[1
]}

机构：

[1] KAROLINSKA INST, INST ENVIRONM MED, DIV TOXICOL, S-17177 STOCKHOLM, SWEDEN

来源：

EXPERIMENTAL CELL RESEARCH | 1994年 / 212卷 / 01期

关键词：

D O I：

10.1006/excr.1994.1121

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The endoplasmic reticular Ca2+-ATPase inhibitor, thapsigargin, was used to study the role of an increase in cytosolic free calcium concentration ([Ca2+](i)) as a signal for the activation of thymocyte apoptosis. Treatment of rat thymocytes with thapsigargin resulted in an early sustained increase in [Ca2+](i) followed by extensive DNA fragmentation. Agarose gel electrophoresis revealed that the pattern of DNA fragments was typical of endonuclease-mediated internucleosomal cleavage. In addition, confocal,microscopy studies showed the formation of apoptotic nuclei in thapsigargin-treated thymocytes. The concentrations of thapsigargin required to induce DNA fragmentation and [Ca2+](i) increase in thymocytes were identical and so were the kinetics of thapsigargin-induced DNA fragmentation and formation of apoptotic nuclei. The lowest concentration of

引用

页码：84 / 92

页数：9

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