HIGH-LEVEL SECRETION OF THE 4 SALIVARY PLASMINOGEN ACTIVATORS FROM THE VAMPIRE BAT DESMODUS-ROTUNDUS BY STABLY TRANSFECTED BABY HAMSTER-KIDNEY CELLS

被引：17

作者：

KRATZSCHMAR, J ^{[1
]}

HAENDLER, B ^{[1
]}

BRINGMANN, P ^{[1
]}

DINTER, H ^{[1
]}

HESS, H ^{[1
]}

DONNER, P ^{[1
]}

SCHLEUNING, WD ^{[1
]}

机构：

[1] SCHERING AG,INST CELLULAR & MOLEC BIOL,RES LABS,POSTFACH 650311,W-1000 BERLIN 65,GERMANY

来源：

GENE | 1992年 / 116卷 / 02期

关键词：

RECOMBINANT DNA; MAMMALIAN EXPRESSION VECTOR; COMBINED SELECTION; PUROMYCIN RESISTANCE; FIBRINOLYSIS; SERINE PROTEASE;

D O I：

10.1016/0378-1119(92)90526-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cDNAs coding for the four Desmodus rotundus salivary plasminogen activators (DSPAs) were subcloned into the mammalian expression vector, pMPSV/CMV, which carries the myeloproliferative sarcoma virus promoter and the cytomegalovirus enhancer. These constructs were transfected, together with plasmids harbouring Geneticin (G418)-resistance and puromycin-resistance genes, into baby hamster kidney cells. Through the selective pressure of both antibiotics, cell clones constitutively overexpressing the DSPA-alpha-1, DSPA-alpha-2, DSPA-beta or DSPA-gamma cDNAs were obtained. Secretion of active DSPAs was confirmed by zymographic analysis and quantified using a fibrin plate assay and ELISA.

引用

页码：281 / 284

页数：4

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