THE EFFECT OF T-LYMPHOCYTE CLONALITY ON THE CALCULATED HPRT MUTATION FREQUENCY OCCURRING IN-VIVO IN HUMANS

被引：37

作者：

ONEILL, JP ^{[1
]}

NICKLAS, JA ^{[1
]}

HUNTER, TC ^{[1
]}

BATSON, OB ^{[1
]}

ALLEGRETTA, M ^{[1
]}

FALTA, MT ^{[1
]}

BRANDA, RF ^{[1
]}

ALBERTINI, RJ ^{[1
]}

机构：

[1] UNIV VERMONT,DEPT MED,GENET LAB,BURLINGTON,VT 05401

来源：

MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS | 1994年 / 313卷 / 2-3期

关键词：

HUMAN T-LYMPHOCYTE; HPRT MUTATION; T-LYMPHOCYTE CLONALITY; T-CELL RECEPTOR GENE REARRANGEMENT; IN VIVO MUTATION;

D O I：

10.1016/0165-1161(94)90052-3

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The frequency of 6-thioguanine resistant (TG(r)) mutant T-lymphocytes arising in vivo in humans can be quantified with a cell cloning assay. However, the in vivo proliferation of T-lymphocytes that may include TG(r) mutant cells can distort the relationship between mutation events and the resulting frequency of mutant cells. The T-cell receptor (TCR) gene rearrangement pattern of T-cell colonies can be used as an independent measure of clonality. Analysis of T-cell 'clonality' in 413 wild type and 1736 TG(r) mutant isolates from 58 individuals shows that mutant clonality is a frequent occurrence (35/58 individuals = 60.3%). However, a major effect on the mutant frequency corrected for clonality (the calculated 'mutation frequency') was found only in nine samples all of which had mutant frequencies greater than 40 x 10(-6).

引用

页码：215 / 225

页数：11

共 21 条

[1] T-CELL CLONING TO DETECT THE MUTANT 6-THIOGUANINE-RESISTANT LYMPHOCYTES PRESENT IN HUMAN PERIPHERAL-BLOOD [J].