CHARACTERIZATION AND EVALUATION BY P-32 POSTLABELING OF PSORALEN TYPE DNA-ADDUCTS IN HELA-CELLS

被引：3

作者：

GILLARDEAUX, O ^{[1
]}

PERINROUSSEL, O ^{[1
]}

NOCENTINI, S ^{[1
]}

PERIN, F ^{[1
]}

机构：

[1] INST CURIE,BIOL SECT,F-75231 PARIS,FRANCE

来源：

CARCINOGENESIS | 1994年 / 15卷 / 01期

关键词：

D O I：

10.1093/carcin/15.1.89

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Samples of DNA irradiated at 405 and/or 365 mm in the presence of 8-methoxypsoralen (8-MOP) were analysed via a modified postlabelling assay using three hydrolysis enzymes other than those employed previously. These enzymes (deoxyribonucleaseI, venom phosphodiesterase and alkaline phosphatase) liberated 3'-adducted dinucleotide monophosphate instead of the 5'-modified dinucleotide monophosphate normally obtained. The first separation chromatography (D1) of samples irradiated in the presence of 8-MOP showed a single spot above the origin, and the next separation (D2) resolved this spot into two components (spots I and D). Double irradiation experiments in which samples of DNA were first irradiated at 405 mm before being irradiated at 365 nm showed that spot in could be transformed into spot I. The use of 6,4,4'-trimethylangelicin, which induced only photomonoadducts under UVA irradiation, gave only spot II. These two results indicated that spots I and II were respectively due to interstrand cross-links and monoadducts. Dose-effect experiments showed that spots I and II were dose dependent, and low-dose irradiations permitted us to measure one interstrand cross-link and two monoadducts per 10(8) base pairs.

引用

页码：89 / 93

页数：5

共 32 条

[21] A NEW SENSITIVE P-32-POSTLABELING ASSAY BASED ON THE SPECIFIC ENZYMATIC CONVERSION OF BULKY DNA LESIONS TO RADIOLABELED DINUCLEOTIDES AND NUCLEOSIDE 5'-MONOPHOSPHATES [J].