BIOCHEMICAL AND GENETIC CLASSIFICATION OF RIBOFLAVINE DEFICIENT MUTANTS OF SACCHAROMYCES-CEREVISIAE

Twenty six riboflavine deficient mutants of Saccharomyces cerevisiae were isolated. They can be divided by biochemical methods into four classes, accumulating (i) no specific product (S), (ii) 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine (AP), (iii) 5-amino-2,6-dihydroxy-4-ribitylaminopyrimidine (HP), (iv) 6,7-dimethyl-8-ribityllumazine (LU). In genetic experiments six complementation groups were found. Complementation group I is congruent with class S, group II with AP, III and IV with HP and V and VI with LU. In the crosses tested so far, no linkage between complementation groups could be detected by tetrad analysis. Intragenic complementation was found within complementation group V (mutants accumulating LU). © 1969 Springer-Verlag.