CRYSTALLIZATION AND PRELIMINARY STRUCTURE-ANALYSIS OF AN INSECT VIRUS WITH T = 4 QUASI-SYMMETRY - NUDAURELIA-CAPENSIS OMEGA-VIRUS

被引:13
作者
CAVARELLI, J
BOMU, W
LILJAS, L
KIM, S
MINOR, W
MUNSHI, S
MUCHMORE, S
SCHMIDT, T
JOHNSON, JE
HENDRY, DA
机构
[1] PURDUE UNIV, DEPT BIOL SCI, W LAFAYETTE, IN 47907 USA
[2] RHODES UNIV, DEPT MICROBIOL, GRAHAMSTOWN 6140, SOUTH AFRICA
来源
ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE | 1991年 / 47卷
关键词
D O I
10.1107/S0108768190009326
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report the crystallization of Nudaurelia capensis omega-virus, a pathogen of the pine emperor moth. The icosahedral particle has T = 4 quasi-equivalent symmetry and is approximately 410 angstrom in diameter. Triclinic crystals (a = 414.0, b = 410.7, c = 420.1 angstrom, alpha = 59.1, beta = 58.9, gamma = 64.0-degrees) diffracting X-rays to 2.7 angstrom resolution have been analyzed using a partial data set collected at high resolution (2.7 angstrom) using conventional oscillation photography and a more complete data set collected at low resolution (50-9 angstrom) using a Siemens area detector. The pseudo-rhombohedral symmetry of the crystals created significant problems in processing the unaligned oscillation photographs. Successful processing of the films depended on the use of an auto-indexing procedure followed by systematic scaling tests of four, nearly equivalent, reduced cells. The unique cell from the area-detector data collection was identified by scaling the four possible choices with the film data. The particle orientation was determined using the rotation function.
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页码:23 / 29
页数:7
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