MOLECULAR-CLONING OF CDNA-ENCODING THE RAT NEURAL CELL-ADHESION MOLECULE L1 - 2 L1 ISOFORMS IN THE CYTOPLASMIC REGION ARE PRODUCED BY DIFFERENTIAL SPLICING

被引：111

作者：

MIURA, M

KOBAYASHI, M

ASOU, H

UYEMURA, K

机构：

[1] Department of Physiology, Keio University School of Medicine, Shinjukuku, Tokyo, 160

来源：

FEBS LETTERS | 1991年 / 289卷 / 01期

关键词：

NEURON; ADHESION MOLECULE; L1; IMMUNOGLOBULIN SUPERFAMILY; CDNA CLONING;

D O I：

10.1016/0014-5793(91)80915-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and sequenced a full-length cDNA encoding the rat neural cell adhesion molecule L1. The deduced amino acid sequence as a whole shows high homology to mouse L1 sequence. In addition to this complete form of L1, we found an isoform, L1cs, which lacks four animo acid residues (RSLE) in the cytoplasmic domain and probably is derived from the same single L1 gene by tissue-specific alternative splicing. While L1 mRNA was predominantly expressed in the brain, L1cs mRNA was found exclusively in peripheral nervous tissue. Differential splicing in the highly conserved cytoplasmic domain may play an important role in modulating the function of L1 in different cells.

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页码：91 / 95

页数：5

相关论文

共 26 条

[1] DROSOPHILA NEUROGLIAN - A MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY WITH EXTENSIVE HOMOLOGY TO THE VERTEBRATE NEURAL ADHESION MOLECULE L1 [J].

BIEBER, AJ ;

SNOW, PM ;

HORTSCH, M ;

PATEL, NH ;

JACOBS, JR ;

TRAQUINA, ZR ;

SCHILLING, J ;

GOODMAN, CS .

CELL, 1989, 59 (03) :447-460

[2] STRUCTURE OF THE CHICKEN NEURON-GLIA CELL-ADHESION MOLECULE, NG-CAM - ORIGIN OF THE POLYPEPTIDES AND RELATION TO THE IG SUPERFAMILY [J].

BURGOON, MP ;

GRUMET, M ;

MAURO, V ;

EDELMAN, GM ;

CUNNINGHAM, BA .

JOURNAL OF CELL BIOLOGY, 1991, 112 (05) :1017-1029

[3] EXTENSION OF NEURITES ON AXONS IS IMPAIRED BY ANTIBODIES AGAINST SPECIFIC NEURAL CELL-SURFACE GLYCOPROTEINS [J].

CHANG, S ;

RATHJEN, FG ;

RAPER, JA .

JOURNAL OF CELL BIOLOGY, 1987, 104 (02) :355-362

[4] NEURAL CELL-ADHESION MOLECULE - STRUCTURE, IMMUNOGLOBULIN-LIKE DOMAINS, CELL-SURFACE MODULATION, AND ALTERNATIVE RNA SPLICING [J].

CUNNINGHAM, BA ;

HEMPERLY, JJ ;

MURRAY, BA ;

PREDIGER, EA ;

BRACKENBURY, R ;

EDELMAN, GM .

SCIENCE, 1987, 236 (4803) :799-806

[5] THE GENE ENCODING L1, A NEURAL ADHESION MOLECULE OF THE IMMUNOGLOBULIN FAMILY, IS LOCATED ON THE X-CHROMOSOME IN MOUSE AND MAN [J].

DJABALI, M ;

MATTEI, MG ;

NGUYEN, C ;

ROUX, D ;

DEMENGEOT, J ;

DENIZOT, F ;

MOOS, M ;

SCHACHNER, M ;

GORIDIS, C ;

JORDAN, BR .

GENOMICS, 1990, 7 (04) :587-593

[6] BIOSYNTHESIS AND MEMBRANE TOPOGRAPHY OF THE NEURAL CELL-ADHESION MOLECULE L1 [J].

FAISSNER, A ;

TEPLOW, DB ;

KUBLER, D ;

KEILHAUER, G ;

KINZEL, V ;

SCHACHNER, M .

EMBO JOURNAL, 1985, 4 (12) :3105-3113

[7] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[8]

FISCHER G, 1986, J NEUROSCI, V6, P605

[9] ISOLATION AND SEQUENCE OF PARTIAL CDNA CLONES OF HUMAN-L1 - HOMOLOGY OF HUMAN AND RODENT-L1 IN THE CYTOPLASMIC REGION [J].

HARPER, JR ;

PRINCE, JT ;

HEALY, PA ;

STUART, JK ;

NAUMAN, SJ ;

STALLCUP, WB .

JOURNAL OF NEUROCHEMISTRY, 1991, 56 (03) :797-804

[10]

HIRANO S, 1987, J CELL BIOL, V106, P873