EXTRACTION AND CHARACTERIZATION OF NEWLY SYNTHESIZED RNA FROM WHOLE CELLS AND CELLULAR FRACTIONS OF RHYNCHOSCIARA ANGELAE SALIVARY GLANDS

被引：12

作者：

ARMELIN, HA

MENEGHINI, R

LARA, FJS

机构：

[1] Laboratório de Biologia Molecular, Departamento de Botânica, Faculdade de Filosofia, Ciências e Letras, Universidade de São Paulo

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 190卷 / 02期

关键词：

D O I：

10.1016/0005-2787(69)90086-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An extraction method resulting in nearly optimal conditions for RNA extraction from salivary-gland cells of Rhynchosciara angelae, as well as a fractionation method for these cells which yields one nuclear and two cytoplasmic fractions, is described. Using these methods, RNA synthesis was studied during middle fourth instar. At this time rRNA was preferentially synthesized, and several rRNA precursors were identified. Heavy RNA and RNA with messenger characteristics were also found. The sedimentation characteristics of RNA obtained from several cellular fractions obtained are reported. © 1969.

引用

页码：358 / +

页数：1

共 22 条

[1]

ARMELIN HA, 1969, GENETICS, V61, P351

[2] NUCLEI FROM RAT LIVER - ISOLATION METHOD THAT COMBINES PURITY WITH HIGH YIELD [J].

BLOBEL, G ;

POTTER, VR .

SCIENCE, 1966, 154 (3757) :1662-&

[3] MASS PREPARATION OF NUCLEI FROM LARVAL SALIVARY GLANDS OF DROSOPHILA HYDEI [J].

BOYD, JB ;

BERENDES, HD ;

BOYD, H .

JOURNAL OF CELL BIOLOGY, 1968, 38 (02) :369-&

[4] BEHAVIOR OF POLYTENE CHROMOSOMES OF RHYNCHOSCIARA ANGELAE AT DIFFERENT STAGES OF LARVAL DEVELOPMENT [J].

BREUER, ME ;

PAVAN, C .

CHROMOSOMA, 1955, 7 (04) :371-386

[5] SEDIMENTATION PROPERTIES OF NEWLY SYNTHESIZED RNA FROM ISOLATED NUCLEAR COMPONENTS OF CHIRONOMUS TENTANS SALIVARY GLAND CELLS [J].

EDSTROM, JE ;

DANEHOLT, B .

JOURNAL OF MOLECULAR BIOLOGY, 1967, 28 (02) :331-&

[6] BIOSYNTHESIS OF MESSENGER AND RIBOSOMAL RIBONUCLEIC ACIDS IN NUCLEOLOCHROMOSOMAL APPARATUS OF ANIMAL CELLS [J].

GEORGIEV, GP ;

SMIRNOV, MN ;

SAMARINA, OP ;

SEVERTZOV, AN ;

LERMAN, MI .

NATURE, 1963, 200 (491) :1291-+

[7] METHYLATION AND PROCESSING OF RIBOSOMAL RNA IN HELA CELLS [J].

GREENBERG, H ;

PENMAN, S .

JOURNAL OF MOLECULAR BIOLOGY, 1966, 21 (03) :527-+

[8]

GREENBERG JR, 1967, J CELL BIOL, V35, pA49

[9]

GUARACIABA H L B, 1967, Revista Brasileira de Biologia, V27, P321

[10] TEMPLATE AND DNA-LIKE RIBONUCLEIC ACIDS AS DISTINCT ENTITIES IN A PREPARATION FROM RAT LIVER [J].

HADJIVAS.A ;

BRAWERMA.G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 103 (02) :211-+

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