EXTRACTION AND CHARACTERIZATION OF NEWLY SYNTHESIZED RNA FROM WHOLE CELLS AND CELLULAR FRACTIONS OF RHYNCHOSCIARA ANGELAE SALIVARY GLANDS

被引：12

作者：

ARMELIN, HA

MENEGHINI, R

LARA, FJS

机构：

[1] Laboratório de Biologia Molecular, Departamento de Botânica, Faculdade de Filosofia, Ciências e Letras, Universidade de São Paulo

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 190卷 / 02期

关键词：

D O I：

10.1016/0005-2787(69)90086-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An extraction method resulting in nearly optimal conditions for RNA extraction from salivary-gland cells of Rhynchosciara angelae, as well as a fractionation method for these cells which yields one nuclear and two cytoplasmic fractions, is described. Using these methods, RNA synthesis was studied during middle fourth instar. At this time rRNA was preferentially synthesized, and several rRNA precursors were identified. Heavy RNA and RNA with messenger characteristics were also found. The sedimentation characteristics of RNA obtained from several cellular fractions obtained are reported. © 1969.

引用

页码：358 / +

页数：1

共 22 条

[11]

LARA F. J. S., 1965, AMER NATUR, V99, P189, DOI 10.1086/282366

[12]

LARA FJS, 1967, 27 NAT CANC I MON, P235

[13]

MARTIN RG, 1961, J BIOL CHEM, V236, P1372

[14] SEQUENCE OF PUFF FORMATION IN RHYNCHOSCIARA POLYTENE CHROMOSOMES [J].

MATTINGL.E ;

PARKER, C .

CHROMOSOMA, 1968, 23 (03) :255-&

[15] CHANGE IN PATTERNS OF INHIBITION BY ACTINOMYCIN D OF URIDINE-H3 INCORPORATION INTO SALIVARY GLAND RNA OF RHYNCHOSCIARA AT DIFFERENT LARVAL AGES [J].

MENEGHINI, R ;

ARMELIN, HA ;

LARA, FJS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 32 (05) :846-+

[16]

PAVAN C, 1959, 10 P INT C GEN MONTR, V1, P321

[17] POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA [J].

PENMAN, S ;

DARNELL, JE ;

SCHERRER, K ;

BECKER, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1963, 49 (05) :654-&

[18] A SYSTEM IN VITRO FOR SYNTHESIS OF RNA AND PROTEIN BY ISOLATED SALIVARY GLANDS AND BY NUCLEI FROM CHIRONOMUS LARVAE [J].

RISTOW, H ;

ARENDS, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 157 (01) :178-&

[19] SEDIMENTATION CHARACTERISTICS OF RAPIDLY LABELLED RNA FROM HELA CELLS [J].

SCHERRER, K ;

DARNELL, JE .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1962, 7 (06) :486-&

[20] PATTERNS OF RNA METABOLISM IN A DIFFERENTIATED CELL - A RAPIDLY LABELED UNSTABLE 60S RNA WITH MESSENGER PROPERTIES IN DUCK ERYTHROBLASTS [J].

SCHERRER, K ;

MARCAUD, L ;

ZAJDELA, F ;

LONDON, IM ;

GROS, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 56 (05) :1571-&

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