AUTONOMOUSLY REPLICATING SINGLE-COPY EPISOMES IN TRYPANOSOMA-BRUCEI SHOW UNUSUAL STABILITY

被引：53

作者：

PATNAIK, PK

KULKARNI, SK

CROSS, GAM

机构：

[1] Laboratory of Molecular Parasitology, The Rockefeller University, New York, NY 10021-6399

来源：

EMBO JOURNAL | 1993年 / 12卷 / 06期

关键词：

ARS ELEMENT; DNA REPLICATION; KINETOPLASTID; SHUTTLE VECTOR;

D O I：

10.1002/j.1460-2075.1993.tb05908.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have obtained several autonomously replicating plasmids in procyclic Trypanosoma brucei. Two of these have been analyzed in detail. Both exist as monomeric single-copy episomes, which nevertheless demonstrate significant stability. These episomes transform procyclics at frequencies that are at least 60-fold higher than those achieved earlier with non-replicating molecules. A modified version of one of these episomes is able to replicate autonomously in bloodstream form T. brucei but it is unstable in this developmental stage of the organism. These episomes will prove useful as shuttle vectors, in functional complementation studies, in the construction of promoter-traps for the isolation of transcriptional promoters in T.brucei, and importantly, as models for the analysis of DNA replication in this ancient eukaryotic lineage.

引用

页码：2529 / 2538

页数：10

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