MULTIPLE UBIQUITIN CONJUGATES ARE PRESENT IN RAT-BRAIN SYNAPTIC-MEMBRANES AND POSTSYNAPTIC DENSITIES

被引：31

作者：

CHAPMAN, AP

SMITH, SJ

RIDER, CC

BEESLEY, PW

机构：

[1] UNIV LONDON,ROYAL HOLLOWAY & BEDFORD NEW COLL,DEPT BIOCHEM,EGHAM TW20 0EX,SURREY,ENGLAND

[2] SMITHKLINE BEECHAM PHARMACEUT,DEPT NEUROL,HARLOW,ESSEX,ENGLAND

来源：

NEUROSCIENCE LETTERS | 1994年 / 168卷 / 1-2期

关键词：

UBIQUITIN; GLYCOPROTEIN; POSTSYNAPTIC DENSITY; SYNAPTIC MEMBRANE; PLASMA MEMBRANE; MONOCLONAL ANTIBODY;

D O I：

10.1016/0304-3940(94)90459-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The pattern of ubiquitin-protein conjugates present in a range of adult rat forebrain subcellular fractions has been investigated by immunoblotting with a monoclonal antibody specific for ubiquitin and its conjugates. Each fraction contains a complex and characteristic pattern of ubiquitin conjugates. Many integral synaptic membrane proteins are ubiquitinated, including a subset of high M(r)(> 120 kD) concanavalin A-binding glycoproteins. Postsynaptic densities are also enriched in ubiquitin conjugates, the profile being distinct from that of synaptic membranes. These results suggest that many plasma membrane and synaptic proteins are ubiquitinated.

引用

页码：238 / 242

页数：5

共 21 条

[1] CHARACTERIZATION OF GP-50, A MAJOR GLYCOPROTEIN PRESENT IN RAT-BRAIN SYNAPTIC-MEMBRANES, WITH A MONOCLONAL-ANTIBODY [J].