EXISTENCE OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C IN RAT-LIVER NUCLEI AND ITS CHANGE DURING LIVER-REGENERATION

被引：55

作者：

KURIKI, H

TAMIYAKOIZUMI, K

ASANO, M

YOSHIDA, S

KOJIMA, K

NIMURA, Y

机构：

[1] NAGOYA UNIV,SCH MED,DIS MECHAN & CONTROL RES INST,CANC CELL BIOL LAB,SHOWA KU,NAGOYA,AICHI 466,JAPAN

[2] NAGOYA UNIV,SCH MED,DEPT SURG 1,SHOWA KU,NAGOYA,AICHI 466,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1992年 / 111卷 / 03期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123750

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We found phosphoinositide-specific phospholipase C (PtdIns-PLC) activity in nuclei isolated from rat liver. The enzyme hydrolyzed phosphatidylinositol, phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol 4,5-bisphosphate in a Ca2+-dependent manner, and produced inositol mono-, bis-, and triphosphate, respectively. Neither phosphatidylcholine, phosphatidylethanolamine, nor phosphatidylserine was utilized as a substrate. After partial hepatectomy, the PtdIns-PLC activity in isolated nuclei increased transiently in the S phase (20-22 h post-hepatectomy), to 2.5-fold higher than in the control, when measured with PIP. This result suggests a close relationship between the nuclear PtdIns-PLC, especially its PIP-hydrolyzing activity, and cell proliferation.

引用

页码：283 / 286

页数：4

共 32 条

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