DETECTION AND IDENTIFICATION OF CAMPYLOBACTER-COLI AND CAMPYLOBACTER-JEJUNI BY 2-STEP POLYMERASE CHAIN-REACTION

被引：5

作者：

COMI, G ^{[1
]}

FERRONI, P ^{[1
]}

COCOLIN, L ^{[1
]}

CANTONI, C ^{[1
]}

MANZANO, M ^{[1
]}

机构：

[1] UNIV UDINE,DEPT FOOD SCI,I-33100 UDINE,ITALY

来源：

MOLECULAR BIOTECHNOLOGY | 1995年 / 3卷 / 03期

关键词：

CAMPYLOBACTER; POLYMERASE CHAIN REACTION; FLAGELLIN GENE;

D O I：

10.1007/BF02789337

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Flagellin gene was used as target sequence to detect and distinguish C. coli and C. jejuni by a ''nested PCR'' technique. The method shows a high level of sensitivity and specificity. Application of this rapid diagnostic tool could provide further information about epidemiological and pathogenetic implications of each of these two microorganisms.

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收藏

页码：266 / 268

页数：3

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