DISTRIBUTION OF ALPHA-TOCOPHEROL IN BEEF HEART MITOCHONDRIA

被引：56

作者：

OLIVEIRA, MM

WEGLICKI, WB

NASON, A

NAIR, PP

机构：

[1] McCollum Pratt Institute, Johns Hopkins University, Baltimore

[2] Gerontology Research Center, National Institute of Child Health and Human Development, Baltimore

[3] Biochemistry Research Division, Department of Medicine, Sinai Hospital of Baltimore, Baltimore

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 180卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0005-2728(69)90197-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. α-Tocopherol was identified and determined in purified bovine heart mitochondria by several independent procedures including thin-layer chromatography, silicic acid column chromatography, gas-liquid chromatography and spectral examination. 2. The concentration of tocopherol in purified mitochondrial preparations ranged from 0.05 to 0.44 μmole of tocopherol per g of protein, and appeared to be independent of the specific activity of cytochrome oxidase in the same samples. 3. α-Tocopherol was found to be present in all the enzymatically active lipoprotein complexes of the electron transport system of mitochondria, but apparently did not have a direct quantitative relationship with the enzymatic activities observed. The highest concentration of tocopherol per g of protein occurred in a supernatant fraction free of any electron transport chain activity. 4. The subcellular fractions of beef heart muscle were examined for vitamin E content, and the crude nuclear fraction showed a considerable amount of tocopherol per g of protein. The supernatant fraction obtained after sedimentation of the mitochondria had very little vitamin E. 5. Separation of the mitochondrial membranes by digitonin treatment showed that only the inner membranes contained tocopherol. © 1969.

引用

页码：98 / &

共 28 条

[1] INNER MEMBRANE AS SITE OF IN VITRO INCORPORATION OF L-[14C]LEUCINE INTO MITOCHONDRIAL PROTEIN [J].