ULTRASTRUCTURAL-LOCALIZATION OF DYSTROPHIN IN CHICKEN SMOOTH-MUSCLE

被引：8

作者：

HARRICANE, MC ^{[1
]}

AUGIER, N ^{[1
]}

LEGER, J ^{[1
]}

ANOAL, M ^{[1
]}

CAVADORE, C ^{[1
]}

MORNET, D ^{[1
]}

机构：

[1] FAC PHARM MONTPELLIER,INSERM,U300,F-34060 MONTPELLIER 1,FRANCE

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1991年 / 15卷 / 08期

关键词：

D O I：

10.1016/0309-1651(91)90069-U

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated the presence of dystrophin in gizzard smooth muscle by immunofluorescence assay, immunoblot detection and an immunogold electron microscopy technique. Western blot analyses, using antibodies raised against sequences 1173-1728 and 3357-3660 of the dystrophin molecule, revealed the presence of a major intact 400 kDa protein band and an immunofluorescence localization restricted to the periphery of the smooth muscle cells. We were able to precisely determine the dystrophin distribution along the plasmalemma whereas caldesmon molecules were present in the cytoplasm. The most commonly observed distance between two neighbouring dystrophin molecules suggested a self-associating arrangement. We discuss these findings in relation to the function of dystrophin in the smooth muscle cell structure. © 1991.

引用

页码：687 / 697

页数：11

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