STUDIES ON PURIFICATION AND CHARACTERIZATION OF PITUITARY THYROTROPIN FROM BLACK SILVER CARP, ARISTICHTHYS-NOBILIS

被引：5

作者：

BANERJEE, S ^{[1
]}

BANERJEE, PP ^{[1
]}

SHEN, ST ^{[1
]}

YU, JYL ^{[1
]}

机构：

[1] ACAD SINICA,INST ZOOL,ENDOCRINOL LAB,TAIPEI 115,TAIWAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1994年 / 107卷 / 02期

关键词：

THYROTROPIN; ARISTICHTHYS NOBILIS;

D O I：

10.1016/0305-0491(94)90057-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thyrotropin (TSH) was isolated from pituitaries of black silver carp (Aristichthys nobilis), designated as bscTSH. Molecular weight of bscTSH was 28 kDa as determined by gel-filtration chromatography. SDS-PAGE analysis revealed the bscTSH consisted of two subunits. Isoelectric point of bscTSH was at pHs from 7.8 to 8.4, and its sialic acid content was 0.86%. Amino acid composition of intact bscTSH was comparable to tetrapod TSHs. The isolated bscTSH was potent in stimulation of the circulating thyroxine level in common carp and loach, but was inactive in stimulation of their gonadal steroidogenesis.

引用

页码：337 / 346

页数：10

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