MODIFIED PURIFICATION OF L-AROMATIC AMINO-ACID DECARBOXYLASE FROM PIG-KIDNEY

被引：3

作者：

DOMINICI, P

MOORE, PS

VOLTATTORNI, CB

机构：

[1] UNIV CATTOLICA SACRO CUORE, FAC MED & CHIRURG A GEMELLI, IST CHIM BIOL, LARGO F VITO, I-00168 ROME, ITALY

[2] UNIV VERONA, FAC MED & CHIRURG, IST CHIM BIOL, I-37134 VERONA, ITALY

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1993年 / 4卷 / 04期

关键词：

D O I：

10.1006/prep.1993.1045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new protocol for purification of L-aromatic amino acid decarboxylase from pig kidney in which, after an initial ultracentrifugation, only two columns are required to obtain homogeneous enzyme is presented. The protocol provides protein with a higher specific activity than that previously obtained, reducing by about one-half the time required for purification. © 1993 Academic Press. All rights reserved.

引用

页码：345 / 347

页数：3

共 17 条

[1] INHIBITION OF PIG-KIDNEY L-AROMATIC AMINO-ACID DECARBOXYLASE BY 2,3-METHANO-META-TYROSINES [J].