RECOVERIN MEDIATES THE CALCIUM EFFECT UPON RHODOPSIN PHOSPHORYLATION AND CGMP HYDROLYSIS IN BOVINE RETINA ROD CELLS

被引：53

作者：

GORODOVIKOVA, EN ^{[1
]}

GIMELBRANT, AA ^{[1
]}

SENIN, II ^{[1
]}

PHILIPPOV, PP ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,AN BELOZERSKY INST PHYSICOCHEM BIOL,DEPT ENZYMOL,MOSCOW 119899,RUSSIA

来源：

FEBS LETTERS | 1994年 / 349卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

PHOTORECEPTION; PHOSPHORYLATION; RHODOPSIN; RHODOPSIN KINASE; RECOVERIN; BOVINE ROD;

D O I：

10.1016/0014-5793(94)00661-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhodopsin phosphorylation and in consequence cGMP hydrolysis in bovine rod outer segments are Ca2+ dependent in the presence of ATP. The level of rhodopsin phosphorylation decreases and the lifetime of active phosphodiesterase increases when the free [Ca2+] is raised from <1 nM to about 1 mu M; in both cases the half-maximal effect was observed at 140-170 nM of free Ca2+. Antibodies to recoverin reverse both effects at high [Ca2+] but have no influence at low [Ca2+]. We conclude that the Ca2+ effects observed are mediated by recoverin which inhibits rhodopsin kinase at a high Ca2+ level.

引用

页码：187 / 190

页数：4

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